Coding

Part:BBa_K3146004:Design

Designed by: Milva Khandakar   Group: iGEM19_Westminster_UK   (2019-10-08)

CRP

cAMP receptor protein involved in the iron homeostasis. The expression of many anaerobic respiratory genes in MR-1, including those for the reduction of fumarate, dimethyl sulfoxide, and metal oxides, is regulated by cyclic AMP receptor protein (CRP). CRP i coordinately regulates the expression of catabolic and respiratory pathways in MR-1, including d-lactate dehydrogenase and anaerobic terminal reductases. CRP directly binds to the upstream regions of omcA and mtrC in the presence of cAMP, and activates the transcription of these metal-reduction genes.


_NOTOC__

cAMP regulatory protein (CRP)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 27
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

prefix and suffix added, illegal sites removed using benchling, twist bioscience and geneious


Source

uniprot -https://www.uniprot.org/uniprot/B8CTF4

References